More than 100 Cuban business owners have appealed to US President-elect Donald Trump not to reverse a recent thaw in bilateral relations.
In a letter, they said additional measures to boost travel, trade and investment would benefit both nations.
Mr. Trump, who takes office on January 20, has said he will end the deal under which ties were restored in 2015 unless Havana offers a "better deal".
Cuba hopes to sign 12 agreements with the US before Mr. Trump's inauguration.
Cuban and US government officials on Wednesday held talks in Havana to discuss how this could be achieved during President Barack Obama's remaining weeks in office.
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